Andreas Scorilas1,2, Anders Bjartell3, Hans Lilja3, Christina Moller3 and Eleftherios P. Diamandis1,2,a
+ Author Affiliations
1Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, M5G 1X5 Canada.
2Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, M5G 1X5 Canada.
3Department of Clinical Chemistry, Lund University, University Hospital Malmo, Malmo S-205 02, Sweden.
↵aAddress correspondence to this author at: Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, 600 University Ave., Toronto, Ontario, M5G 1X5 Canada. Fax 416-586-8628; e-mail ediamandis@mtsinai.on.ca.
Tuesday 27 December 2011
Streptavidin-Polyvinylamine Conjugates Labeled with a Europium Chelate: Applications in Immunoassay, Immunohistochemistry, and Microarrays
Streptavidin Citation - 2010
L Wang, Y Zhang, X Gao, Z Duan… - Journal of agricultural …, 2010 - ACS Publications
A sensitive biotin− streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA)
method was developed for the determination of chloramphenicol residues in milk. The
biotin− streptavidin system was applied to enhance the sensitivity. After optimization, the ...
Cited by 19 - Related articles - All 4 versions
A sensitive biotin− streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA)
method was developed for the determination of chloramphenicol residues in milk. The
biotin− streptavidin system was applied to enhance the sensitivity. After optimization, the ...
Cited by 19 - Related articles - All 4 versions
[HTML] from mcponline.orgHYH Kim, KA Tallman, DC Liebler… - Molecular & Cellular …, 2009 - ASBMB
Abstract HNE (4-hydroxynonenal), a byproduct of lipid peroxidation, reacts with nucleophilic
centers on proteins. A terminal alkynyl analog of HNE (alkynyl HNE, aHNE) serves as a
surrogate for HNE itself, both compounds reacting with protein amine and thiol functional ...
Cited by 11 - Related articles - All 8 versions
Abstract HNE (4-hydroxynonenal), a byproduct of lipid peroxidation, reacts with nucleophilic
centers on proteins. A terminal alkynyl analog of HNE (alkynyl HNE, aHNE) serves as a
surrogate for HNE itself, both compounds reacting with protein amine and thiol functional ...
Cited by 11 - Related articles - All 8 versions
[PDF] from hokudai.ac.jpR Kawamura, A Kakugo, Y Osada… - Nanotechnology, 2010 - iopscience.iop.org
Recently, a method was established for the formation of microtubule (MT) assemblies by an
active self-organization (AcSO) process, in which MTs were crosslinked during sliding
motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate ( ...
Cited by 11 - Related articles - All 11 versions
Recently, a method was established for the formation of microtubule (MT) assemblies by an
active self-organization (AcSO) process, in which MTs were crosslinked during sliding
motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate ( ...
Cited by 11 - Related articles - All 11 versions
[HTML] from nih.govCE Chivers, E Crozat, C Chu, VT Moy… - Nature methods, 2010 - nature.com
Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur,
limiting its use in imaging, DNA amplification and nanotechnology. We identified a mutant
streptavidin, traptavidin, with more than tenfold slower biotin dissociation, increased ...
Cited by 8 - Related articles - All 9 versions
Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur,
limiting its use in imaging, DNA amplification and nanotechnology. We identified a mutant
streptavidin, traptavidin, with more than tenfold slower biotin dissociation, increased ...
Cited by 8 - Related articles - All 9 versions
[HTML] from nih.govHA Ebhardt… - RNA, 2009 - rnajournal.cshlp.org
Abstract Here we present a simple and inexpensive gel-shift assay for the detection and
quantification of small RNAs. The assay is at least 5–10 times more sensitive than a
conventional Northern, and is highly scalable. Total RNA is first size purified to enrich the ...
Cited by 4 - Related articles - All 11 versions
Abstract Here we present a simple and inexpensive gel-shift assay for the detection and
quantification of small RNAs. The assay is at least 5–10 times more sensitive than a
conventional Northern, and is highly scalable. Total RNA is first size purified to enrich the ...
Cited by 4 - Related articles - All 11 versions
[CITATION] The C2-streptavidin delivery system promotes the uptake of biotinylated molecules in macrophages and T-leukemia cells
Cited by 4 - Related articles - All 3 versions
[PDF] from abbs.infoC Wang, G Yang, Z Luo… - Acta biochimica et …, 2009 - abbs.oxfordjournals.org
Abstract In this study, we developed a systematic evolution of ligands by exponential
enrichment (SELEX) method using a combination of magnetic beads immobilization and
flow cytometric measurement. As an example, the selection of streptavidin-specific ...
Cited by 3 - Related articles - All 8 versions
Abstract In this study, we developed a systematic evolution of ligands by exponential
enrichment (SELEX) method using a combination of magnetic beads immobilization and
flow cytometric measurement. As an example, the selection of streptavidin-specific ...
Cited by 3 - Related articles - All 8 versions
[HTML] from nih.govL Baugh, I Le Trong, DS Cerutti, S Gülich… - Biochemistry, 2010 - ACS Publications
We have identified a distal point mutation in streptavidin that causes a 1000-fold reduction in
biotin binding affinity without disrupting the equilibrium complex structure. The F130L
mutation creates a small cavity occupied by a water molecule; however, all neighboring ...
Cited by 3 - Related articles - All 4 versions
We have identified a distal point mutation in streptavidin that causes a 1000-fold reduction in
biotin binding affinity without disrupting the equilibrium complex structure. The F130L
mutation creates a small cavity occupied by a water molecule; however, all neighboring ...
Cited by 3 - Related articles - All 4 versions
T Tanaka, S Masunari, J Ishii, K Wakamura… - Journal of …, 2010 - Elsevier
Here we expand the yeast cell surface display system to display non-natural, functional
molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli
(BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through ...
Cited by 2 - Related articles - All 3 versions
Here we expand the yeast cell surface display system to display non-natural, functional
molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli
(BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through ...
Cited by 2 - Related articles - All 3 versions
[HTML] from rsmjournals.comVHB Ho, KH Müller, NJ Darton… - Experimental …, 2009 - ebm.rsmjournals.com
Abstract A simple methodology for cell patterning has been developed that can potentially
be used to position different types of mammalian cells with high precision. In this method,
cell membrane proteins were first biotinylated and then bound to streptavidin ...
Cited by 3 - Related articles - All 7 versions
Abstract A simple methodology for cell patterning has been developed that can potentially
be used to position different types of mammalian cells with high precision. In this method,
cell membrane proteins were first biotinylated and then bound to streptavidin ...
Cited by 3 - Related articles - All 7 versions
JG Bruno - In Vitro Cellular & Developmental Biology-Animal, 2010 - Springer
Abstract Nucleic acid aptamers are regarded as rivals for antibodies and as such are being
investigated for their therapeutic potential. In the present work, it is shown that two different
high-affinity DNA aptamers developed previously by Ferreira et al. against MUC1 antigen ...
Cited by 2 - Related articles - All 5 versions
Abstract Nucleic acid aptamers are regarded as rivals for antibodies and as such are being
investigated for their therapeutic potential. In the present work, it is shown that two different
high-affinity DNA aptamers developed previously by Ferreira et al. against MUC1 antigen ...
Cited by 2 - Related articles - All 5 versions
T Kudo, M Ueda, H Konishi, H Kawashima… - Molecular Imaging and …, 2010 - Springer
Abstract Purpose We aimed to evaluate the feasibility of using streptavidin–biotin-based
pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor
(HIF)-1-active tumors. Procedures We used POS, a genetically engineered form of ...
Cited by 3 - Related articles - All 3 versions
Abstract Purpose We aimed to evaluate the feasibility of using streptavidin–biotin-based
pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor
(HIF)-1-active tumors. Procedures We used POS, a genetically engineered form of ...
Cited by 3 - Related articles - All 3 versions
SP Rad'ko, SA Voronina, AV Gromov… - Bulletin of experimental …, 2009 - Springer
Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied
by the surface plasmon resonance method. Conjugation of the probe with streptavidin and
gold nanoparticles was shown to amplify the signal at similar concentrations of this probe ( ...
Cited by 1 - Related articles - All 5 versions
Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied
by the surface plasmon resonance method. Conjugation of the probe with streptavidin and
gold nanoparticles was shown to amplify the signal at similar concentrations of this probe ( ...
Cited by 1 - Related articles - All 5 versions
K Hensley - Methods in molecular biology (Clifton, NJ), 2009 - Springer
Summary Oxidative posttranslational protein modifications occur as a normal process of cell
biology and to a greater extent during pathogenic conditions. The detection and quantitation
of protein oxidation has posed a continuing challenge to bioanalytical chemists because ...
Cited by 1 - Related articles - All 2 versions
Summary Oxidative posttranslational protein modifications occur as a normal process of cell
biology and to a greater extent during pathogenic conditions. The detection and quantitation
of protein oxidation has posed a continuing challenge to bioanalytical chemists because ...
Cited by 1 - Related articles - All 2 versions
MA Zhi-hong - Journal of Anhui Agricultural Sciences, 2009 - en.cnki.com.cn
Based on biotin-streptavidin enzyme-linked immunosorbent assay (ELISA), the detection
method on zearalenone ZEN-ELISA was established. The detection sensitivity of this
method was 0.8 ng/ml, and the variation coefficients of intra-assay and inter-assay were ...
Cited by 1 - Related articles - Cached
Based on biotin-streptavidin enzyme-linked immunosorbent assay (ELISA), the detection
method on zearalenone ZEN-ELISA was established. The detection sensitivity of this
method was 0.8 ng/ml, and the variation coefficients of intra-assay and inter-assay were ...
Cited by 1 - Related articles - Cached
CK Hsu… - Journal of Molecular Graphics and Modelling, 2010 - Elsevier
Wt streptavidin forms a domain swapped tetramer consisting of two native dimers. The role
of tetramerization has been studied previously and is known to contribute to biotin binding
by allowing the exchange of W120 between adjacent subunits. However, the role of dimer ...
Cited by 1 - Related articles - All 3 versions
Wt streptavidin forms a domain swapped tetramer consisting of two native dimers. The role
of tetramerization has been studied previously and is known to contribute to biotin binding
by allowing the exchange of W120 between adjacent subunits. However, the role of dimer ...
Cited by 1 - Related articles - All 3 versions
L No - img.creative-biomart.com
1. Howarth M., et al. (2006) Nat Methods. 3(4):267-73. 2. Holmberg A., et al (2005).
Electrophoresis. 26(3):501-10. ... Streptomyces avidinii, Recombinant(Streptavidin,
His-tagged) Expressed in E.coli Cat. No. Streptavidin-390S Lot. No. (See product label)
Related articles - View as HTML
1. Howarth M., et al. (2006) Nat Methods. 3(4):267-73. 2. Holmberg A., et al (2005).
Electrophoresis. 26(3):501-10. ... Streptomyces avidinii, Recombinant(Streptavidin,
His-tagged) Expressed in E.coli Cat. No. Streptavidin-390S Lot. No. (See product label)
Related articles - View as HTML
[PDF] B3 (Fab)-streptavidin Tetramer Has Higher Binding Avidity than B3 (scFv)-streptavidin Tetramer
Multivalent and multi-specific antibodies can provide valuable tools for bio-medical
research, diagnosis and therapy. In antigen-antibody interactions, the avidity of antibodies
depends on the affinity and the number of binding sites. 1 As artificial multivalent antibody ...
Related articles - View as HTML - All 5 versions
CX Xu, ZM Hu, JL Li… - Nan fang yi ke da xue xue bao= …, 2009 - ncbi.nlm.nih.gov
OBJECTIVE: To study the purification, refolding and bioactivity of streptavidin-tagged human
tumor necrosis factor-alpha (SA-TNF-alpha) bi-functional fusion protein. METHODS: SA-TNF-
alpha fusion protein was expressed in BL21 (DE3) host bacteria, purified using Ni-NTA ...
OBJECTIVE: To study the purification, refolding and bioactivity of streptavidin-tagged human
tumor necrosis factor-alpha (SA-TNF-alpha) bi-functional fusion protein. METHODS: SA-TNF-
alpha fusion protein was expressed in BL21 (DE3) host bacteria, purified using Ni-NTA ...
[HTML] from nih.govK Leung - 2009 - ncbi.nlm.nih.gov
Optical fluorescence imaging is increasingly being used to monitor biological functions of
specific targets in small animals (1-3). However, the intrinsic fluorescence of biomolecules
poses a problem when fluorophores that absorb visible light (350–700 nm) are used. Near ...
Related articles - All 3 versions
Optical fluorescence imaging is increasingly being used to monitor biological functions of
specific targets in small animals (1-3). However, the intrinsic fluorescence of biomolecules
poses a problem when fluorophores that absorb visible light (350–700 nm) are used. Near ...
Related articles - All 3 versions
S Yokota - Methods in molecular biology (Clifton, NJ), 2010 - Springer
Abstract Colloidal gold probes, including protein A-, IgG-F (ab') 2-, and streptavidin-labeled
gold particles, are useful tools for localization of antigens in cells and tissues by
immunoelectron microscopy (IEM). This chapter describes different methods for the ...
All 2 versions
Abstract Colloidal gold probes, including protein A-, IgG-F (ab') 2-, and streptavidin-labeled
gold particles, are useful tools for localization of antigens in cells and tissues by
immunoelectron microscopy (IEM). This chapter describes different methods for the ...
All 2 versions
H Su, YL Chen, SY Chen, B Wen… - Nan fang yi ke da xue …, 2009 - ncbi.nlm.nih.gov
OBJECTIVE: To obtain streptavidin-tagged human interleukin-15 (SA/hIL15) fusion protein
and evaluate its bioactivity. METHODS: pET24a-6His-SA-hIL-15 and pET32a-hIL-15-SA-
6His plasmids were constructed and expressed in BL 21 (DE3) host bacteria to generate ...
OBJECTIVE: To obtain streptavidin-tagged human interleukin-15 (SA/hIL15) fusion protein
and evaluate its bioactivity. METHODS: pET24a-6His-SA-hIL-15 and pET32a-hIL-15-SA-
6His plasmids were constructed and expressed in BL 21 (DE3) host bacteria to generate ...
C Oliver - Methods in molecular biology (Clifton, NJ), 2010 - ncbi.nlm.nih.gov
Biotin-avidin detection systems are widely used in both immunocytochemistry and molecular
biology. They take advantage of the high affinity of biotin, a low-molecular-weight vitamin, for
avidin, an egg-white protein. Because of the problem of nonspecific binding of avidin, ...
Biotin-avidin detection systems are widely used in both immunocytochemistry and molecular
biology. They take advantage of the high affinity of biotin, a low-molecular-weight vitamin, for
avidin, an egg-white protein. Because of the problem of nonspecific binding of avidin, ...
F PENG, X CHEN, F XU… - Food Science, 2009 - en.cnki.com.cn
PENG Fu-zhong1, CHEN Xue-lan1,*, XU Feng2, XIONG Yong-hua2 (1. College of Life
Science, Jiangxi Normal University, Nanchang 330022, China; 2. State Key Laboratory of
Food Science and Technology, Nanchang University, Nanchang 330047, China)
Cached
PENG Fu-zhong1, CHEN Xue-lan1,*, XU Feng2, XIONG Yong-hua2 (1. College of Life
Science, Jiangxi Normal University, Nanchang 330022, China; 2. State Key Laboratory of
Food Science and Technology, Nanchang University, Nanchang 330047, China)
Cached
L Wang… - Methods in enzymology, 2010 - Elsevier
Abstract In this chapter, we describe the preparation of cryo-EM specimens for random
spherically constrained (RSC) single-particle reconstruction of membrane proteins. The
specimen consists of liposomes into which the purified membrane protein is reconstituted ...
Related articles - All 5 versions
Abstract In this chapter, we describe the preparation of cryo-EM specimens for random
spherically constrained (RSC) single-particle reconstruction of membrane proteins. The
specimen consists of liposomes into which the purified membrane protein is reconstituted ...
Related articles - All 5 versions
[PDF] from biomedcentral.comJH Kim, T Chang, A Graham, KA Choo… - BMC …, 2010 - biomedcentral.com
Background Cell biologists face the need to rapidly analyse their proteins of interest in order
to gain insight into their function. Often protein purification, cellular localisation and Western
blot analyses can be multi-step processes, where protein is lost, activity is destroyed or ...
Related articles - All 6 versions
Background Cell biologists face the need to rapidly analyse their proteins of interest in order
to gain insight into their function. Often protein purification, cellular localisation and Western
blot analyses can be multi-step processes, where protein is lost, activity is destroyed or ...
Related articles - All 6 versions
PP Fa, Z Zhang, JL Li, ZM Hu… - Nan fang yi ke da xue xue …, 2010 - ncbi.nlm.nih.gov
OBJECTIVE: To obtain streptavidin-tagged human interleukin-21 (hIL21) fusion protein and
evaluate its bioactivities. METHODS: hIL21-SA-pET21 and pET24a-SA-hIL21 plasmids were
constructed and expressed in BL21 (DE3) host bacteria. The hIL21-SA and SA-hIL21 ...
OBJECTIVE: To obtain streptavidin-tagged human interleukin-21 (hIL21) fusion protein and
evaluate its bioactivities. METHODS: hIL21-SA-pET21 and pET24a-SA-hIL21 plasmids were
constructed and expressed in BL21 (DE3) host bacteria. The hIL21-SA and SA-hIL21 ...
AY Golovina, AA Bogdanov, OA Dontsova… - Biochimie, 2010 - Elsevier
Preparation of pure ribosomal subunits carrying lethal mutations is necessary for studying
every essential functional region of ribosomal RNA. Affinity purification via a tag, inserted
into rRNA proved to be procedure of choice for purification of such ribosomal subunits. ...
Related articles - All 5 versions
Preparation of pure ribosomal subunits carrying lethal mutations is necessary for studying
every essential functional region of ribosomal RNA. Affinity purification via a tag, inserted
into rRNA proved to be procedure of choice for purification of such ribosomal subunits. ...
Related articles - All 5 versions
MJ Navakouski, II Vashkevich… - Applied Biochemistry and …, 2009 - Springer
Abstract The effect of precipitation of complexes of streptavidin with biotinylated proteins
under conditions of simple (according to Mancini) and double (according to Ouchterlony)
radial diffusion in agar gel was studied. The position and form of precipitation lines ...
Related articles - All 5 versions
Abstract The effect of precipitation of complexes of streptavidin with biotinylated proteins
under conditions of simple (according to Mancini) and double (according to Ouchterlony)
radial diffusion in agar gel was studied. The position and form of precipitation lines ...
Related articles - All 5 versions
M Park, J Jose, S Thommes, JI Kim… - Enzyme and Microbial …, 2010 - Elsevier
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion
protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse
adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed ...
Related articles - All 3 versions
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion
protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse
adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed ...
Related articles - All 3 versions
[PDF] from roche.comC No - e-labdoc.roche.com
Streptavidin from E. coli K12 was coupled to alkaline phosphatase (AP) from calf intestine
via heterobifunctional linkers. This conjugate was purified using column chromatography.
The final preparation is stabilized in 30 mM triethanolamine buffer, pH 7.6, 0.5% bovine ...
Related articles - All 5 versions
Streptavidin from E. coli K12 was coupled to alkaline phosphatase (AP) from calf intestine
via heterobifunctional linkers. This conjugate was purified using column chromatography.
The final preparation is stabilized in 30 mM triethanolamine buffer, pH 7.6, 0.5% bovine ...
Related articles - All 5 versions
A Agarwal… - Biophysical Journal, 2009 - adsabs.harvard.edu
Title: Velocity-dependence of Cargo Loading onto Molecular Shuttles Demonstrates the
Glue-like Character of Biotin/Streptavidin. Authors: Agarwal, Ashutosh; Hess, Henry. Publication:
Biophysical Journal, vol. 96, issue 3, pp. 317a-317a. Publication Date: 02/2009. ...
All 2 versions
Title: Velocity-dependence of Cargo Loading onto Molecular Shuttles Demonstrates the
Glue-like Character of Biotin/Streptavidin. Authors: Agarwal, Ashutosh; Hess, Henry. Publication:
Biophysical Journal, vol. 96, issue 3, pp. 317a-317a. Publication Date: 02/2009. ...
All 2 versions
T Matsumoto, S Sawamoto, T Sakamoto… - Journal of bioscience …, 2009 - ci.nii.ac.jp
... 論文名, 著者名, 著者所属, 刊行物名, ISSN, 巻, 号, ページ, 出版者, 参考文献, 出版年, 年から 年まで.
すべて CiNiiに本文あり CiNiiに本文あり、または連携サービスへのリンクあり. EP-P27 Site-specific
streptavidin modification using sortase(Section VII Enzyme and Protein Engineering). ...
Cached - All 2 versions
... 論文名, 著者名, 著者所属, 刊行物名, ISSN, 巻, 号, ページ, 出版者, 参考文献, 出版年, 年から 年まで.
すべて CiNiiに本文あり CiNiiに本文あり、または連携サービスへのリンクあり. EP-P27 Site-specific
streptavidin modification using sortase(Section VII Enzyme and Protein Engineering). ...
Cached - All 2 versions
Streptavidin Citation - 2011
TT Nguyen, KL Sly… - Analytical Chemistry, 2011 - ACS Publications
A comparison of the binding properties of avidin, streptavidin, neutrAvidinTM and anti-biotin
antibody to a biotinylated lipid bilayer was studied using second harmonic generation.
Protein binding assays were performed on a planar supported lipid bilayer of 1, 2-dioleoyl ...
A comparison of the binding properties of avidin, streptavidin, neutrAvidinTM and anti-biotin
antibody to a biotinylated lipid bilayer was studied using second harmonic generation.
Protein binding assays were performed on a planar supported lipid bilayer of 1, 2-dioleoyl ...
[CITATION] Identification and Analysis of Protein Adducts of 4Hydroxy-2-nonenal and 4-Oxo-2-nonenal Using a Novel Streptavidin Catch and Photorelease Approach
G Lai, J Wu, H Ju… - Advanced Functional Materials, 2011 - Wiley Online Library
Lai, G., Wu, J., Ju, H. and Yan, F.(2011), Streptavidin-Functionalized Silver-Nanoparticle-
Enriched Carbon Nanotube Tag for Ultrasensitive Multiplexed Detection of Tumor Markers.
Advanced Functional Materials, 21: n/a. doi: 10.1002/adfm. 201100396
Cited by 2 - Related articles - All 4 versions
Lai, G., Wu, J., Ju, H. and Yan, F.(2011), Streptavidin-Functionalized Silver-Nanoparticle-
Enriched Carbon Nanotube Tag for Ultrasensitive Multiplexed Detection of Tumor Markers.
Advanced Functional Materials, 21: n/a. doi: 10.1002/adfm. 201100396
Cited by 2 - Related articles - All 4 versions
L Horling, WL Neuhuber… - Journal of Neuroscience Methods, 2011 - Elsevier
Abstract Highly sensitive immunohistochemical detection systems such as tyramide signal
amplification (TSA) are widely used, since they allow using two primary antibodies raised in
the same species. Most of them are based on the streptavidin-biotin-peroxidase system ...
Abstract Highly sensitive immunohistochemical detection systems such as tyramide signal
amplification (TSA) are widely used, since they allow using two primary antibodies raised in
the same species. Most of them are based on the streptavidin-biotin-peroxidase system ...
L Baugh, I Le Trong, DS Cerutti, N Mehta… - Biochemistry, 2011 - ACS Publications
We report a point mutation in the second contact shell of the high-affinity streptavidin-biotin
complex that appears to reduce binding affinity through transmitted effects on equilibrium
dynamics. The Y54F streptavidin mutation causes a 75-fold loss of binding affinity with 73- ...
We report a point mutation in the second contact shell of the high-affinity streptavidin-biotin
complex that appears to reduce binding affinity through transmitted effects on equilibrium
dynamics. The Y54F streptavidin mutation causes a 75-fold loss of binding affinity with 73- ...
M Kim, CC Wang, F Benedetti, M Rabbi… - Advanced …, 2011 - Wiley Online Library
* Correspondence: Piotr E. Marszalek, Department of Mechanical Engineering and Materials
Science, Center for Biologically Inspired Materials and Material Systems, Center for
Biomolecular and Tissue Engineering, Duke University, Durham, NC 27708, USA.
* Correspondence: Piotr E. Marszalek, Department of Mechanical Engineering and Materials
Science, Center for Biologically Inspired Materials and Material Systems, Center for
Biomolecular and Tissue Engineering, Duke University, Durham, NC 27708, USA.
JD Stone, MN Artyomov, AS Chervin… - The Journal of …, 2011 - Am Assoc Immnol
Abstract The binding of oligomeric peptide–MHC (pMHC) complexes to cell surface TCR
can be considered to approximate TCR–pMHC interactions at cell-cell interfaces. In this
study, we analyzed the equilibrium binding of streptavidin-based pMHC oligomers ( ...
Abstract The binding of oligomeric peptide–MHC (pMHC) complexes to cell surface TCR
can be considered to approximate TCR–pMHC interactions at cell-cell interfaces. In this
study, we analyzed the equilibrium binding of streptavidin-based pMHC oligomers ( ...
O Stanek, I Linhartova, L Majlessi, C Leclerc… - Molecular …, 2011 - Springer
Abstract The choice of tools that enable efficient targeting of exogenous antigens (Ag) for
processing and presentation by professional Ag-presenting cells (APC) remains limited. This
represents, indeed, a bottleneck in development of vaccines inducing specific T-cell ...
Related articles - All 2 versions
Abstract The choice of tools that enable efficient targeting of exogenous antigens (Ag) for
processing and presentation by professional Ag-presenting cells (APC) remains limited. This
represents, indeed, a bottleneck in development of vaccines inducing specific T-cell ...
Related articles - All 2 versions
I Le Trong, Z Wang, DE Hyre… - … Section D: Biological …, 2011 - scripts.iucr.org
Atomic resolution crystallographic studies of streptavidin and its biotin complex have been
carried out at 1.03 and 0.95 A, respectively. The wild-type protein crystallized with a tetramer
in the asymmetric unit, while the crystals of the biotin complex contained two subunits in ...
Related articles - All 3 versions
Atomic resolution crystallographic studies of streptavidin and its biotin complex have been
carried out at 1.03 and 0.95 A, respectively. The wild-type protein crystallized with a tetramer
in the asymmetric unit, while the crystals of the biotin complex contained two subunits in ...
Related articles - All 3 versions
JP Daguer, M Ciobanu, S Barluenga… - Org. Biomol. Chem …, 2011 - pubs.rsc.org
Dehydrocholic acid was identified as a selective streptavidin binder from a PNA-tagged
library. Isothermal calorimetry titration measurements showed this interaction to be
entropically driven. Peptides tagged with dehydrocholic acid can be captured on a ...
Dehydrocholic acid was identified as a selective streptavidin binder from a PNA-tagged
library. Isothermal calorimetry titration measurements showed this interaction to be
entropically driven. Peptides tagged with dehydrocholic acid can be captured on a ...
Y Li, S Franklin, MJ Zhang… - Protein Science, 2011 - Wiley Online Library
Highly efficient purification of protein complexes from mammalian cells using a novel
streptavidin-binding peptide and hexahistidine tandem tag system: Application to Bruton's
tyrosine kinase-Li-2010-Protein Science-Wiley Online Library
Cited by 1 - Related articles - All 3 versions
Highly efficient purification of protein complexes from mammalian cells using a novel
streptavidin-binding peptide and hexahistidine tandem tag system: Application to Bruton's
tyrosine kinase-Li-2010-Protein Science-Wiley Online Library
Cited by 1 - Related articles - All 3 versions
RJ Austin, HM Smidansky, CA Holstein… - …, 2011 - Wiley Online Library
Austin, RJ, Smidansky, HM, Holstein, CA, Chang, DK, Epp, A., Josephson, NC and Martin,
DB (2011), Proteomic analysis of the androgen receptor via MS-compatible purification of
biotinylated protein on streptavidin resin. PROTEOMICS. doi: 10.1002/pmic. 201100348
Austin, RJ, Smidansky, HM, Holstein, CA, Chang, DK, Epp, A., Josephson, NC and Martin,
DB (2011), Proteomic analysis of the androgen receptor via MS-compatible purification of
biotinylated protein on streptavidin resin. PROTEOMICS. doi: 10.1002/pmic. 201100348
[HTML] from nih.govR Mittal… - Bioconjugate Chemistry, 2011 - ACS Publications
The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to
their performance in cell tagging, tracking, and imaging experiments. Commercially
available streptavidin conjugates (SAv QDs) are the most commonly used tool for ...
Cited by 2 - Related articles - All 6 versions
The valency of quantum dot nanoparticles conjugated with biomolecules is closely related to
their performance in cell tagging, tracking, and imaging experiments. Commercially
available streptavidin conjugates (SAv QDs) are the most commonly used tool for ...
Cited by 2 - Related articles - All 6 versions
MLB Magalhães, CM Czekster, R Guan… - Protein …, 2011 - Wiley Online Library
Page 1. Evolved Streptavidin Mutants Reveal Key Role Of Loop Residue In High-Affinity
Binding ... USA. Running head: Analysis of Streptavidin Variants Address correspondence
to: Matthew Levy, 1301 Morris Park Avenue, Price Center, ...
Cited by 1 - Related articles - All 2 versions
Page 1. Evolved Streptavidin Mutants Reveal Key Role Of Loop Residue In High-Affinity
Binding ... USA. Running head: Analysis of Streptavidin Variants Address correspondence
to: Matthew Levy, 1301 Morris Park Avenue, Price Center, ...
Cited by 1 - Related articles - All 2 versions
SI Park, J Shenoi, SL Frayo, DK Hamlin, Y Lin… - Clinical Cancer …, 2011 - AACR
Purpose: Pretargeted radioimmunotherapy (PRIT) using streptavidin (SAv)-biotin technology
can deliver higher therapeutic doses of radioactivity to tumors than conventional RIT.
However," endogenous" biotin can interfere with the effectiveness of this approach by ...
Related articles - All 2 versions
Purpose: Pretargeted radioimmunotherapy (PRIT) using streptavidin (SAv)-biotin technology
can deliver higher therapeutic doses of radioactivity to tumors than conventional RIT.
However," endogenous" biotin can interfere with the effectiveness of this approach by ...
Related articles - All 2 versions
Z Yang, C Zong, H Ju… - Analytica Chimica Acta, 2011 - Elsevier
A streptavidin functionalized capillary immune microreactor was designed for highly efficient
flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be
used as both a support for highly efficient immobilization of antibody and a flow cell for ...
All 2 versions
A streptavidin functionalized capillary immune microreactor was designed for highly efficient
flow-through chemiluminescent (CL) immunoassay. The functionalized capillary could be
used as both a support for highly efficient immobilization of antibody and a flow cell for ...
All 2 versions
[PDF] from mdpi.comKS Rabe… - Molecules, 2011 - mdpi.com
Abstract: Protein-DNA conjugates have found numerous applications in the field of
diagnostics and nanobiotechnology, however, their intrinsic susceptibility to DNA
degradation by nucleases represents a major obstacle for many applications. We here ...
Cited by 1 - Related articles - All 4 versions
Abstract: Protein-DNA conjugates have found numerous applications in the field of
diagnostics and nanobiotechnology, however, their intrinsic susceptibility to DNA
degradation by nucleases represents a major obstacle for many applications. We here ...
Cited by 1 - Related articles - All 4 versions
[PDF] from chem-ju-nju-edu.comW Gao, H Dong, J Lei, H Ji… - Chem. Commun., 2011 - xlink.rsc.org
Abstract A novel biosensing strategy for selective electrochemical detection of DNA down to
the attomolar level with a linear range of 5 orders of magnitude was developed by the
specific recognitions of target DNA and streptavidin to biotin labelled molecular beacon ...
Cited by 2 - Related articles - All 5 versions
Abstract A novel biosensing strategy for selective electrochemical detection of DNA down to
the attomolar level with a linear range of 5 orders of magnitude was developed by the
specific recognitions of target DNA and streptavidin to biotin labelled molecular beacon ...
Cited by 2 - Related articles - All 5 versions
Y Liu, D Cheng, X Liu, G Liu, S Dou… - Nuclear Medicine and …, 2011 - Elsevier
Methods As a test of our hypothesis, the intracellular fate of an antisense oligomer when
incubated as the nanoparticle and when incubated while covalently conjugated to the
antibody was compared. The three-component noncovalent nanoparticle consisted of ...
Related articles - All 3 versions
Methods As a test of our hypothesis, the intracellular fate of an antisense oligomer when
incubated as the nanoparticle and when incubated while covalently conjugated to the
antibody was compared. The three-component noncovalent nanoparticle consisted of ...
Related articles - All 3 versions
R Wilson - nucleic acid therapeutics, 2011 - liebertonline.com
The preparation of single-stranded DNA from double-stranded PCR products is an essential
step in the identification of aptamers by Systematic Evolution of Ligands by EXponential
enrichment (SELEX). The most widely used method for producing single-stranded DNA is ...
Related articles - All 2 versions
The preparation of single-stranded DNA from double-stranded PCR products is an essential
step in the identification of aptamers by Systematic Evolution of Ligands by EXponential
enrichment (SELEX). The most widely used method for producing single-stranded DNA is ...
Related articles - All 2 versions
H Šípová, T Špringer… - Analytical and Bioanalytical Chemistry, 2011 - Springer
Abstract This paper reports an approach to detection of single nucleotide polymorphism
based on special amplification assay and surface plasmon resonance biosensor
technology. In this assay, a part of the target DNA is recognized by a probe (probe A) ...
Cited by 1 - Related articles - All 5 versions
Abstract This paper reports an approach to detection of single nucleotide polymorphism
based on special amplification assay and surface plasmon resonance biosensor
technology. In this assay, a part of the target DNA is recognized by a probe (probe A) ...
Cited by 1 - Related articles - All 5 versions
V Köhler, J Mao, T Heinisch… - Angewandte …, 2011 - Wiley Online Library
Köhler, V., Mao, J., Heinisch, T., Pordea, A., Sardo, A., Wilson, YM, Knörr, L., Creus, M.,
Prost, J.-C., Schirmer, T. and Ward, TR (2011), OsO 4⋅ Streptavidin: A Tunable Hybrid
Catalyst for the Enantioselective cis-Dihydroxylation of Olefins. Angewandte Chemie ...
All 3 versions
Köhler, V., Mao, J., Heinisch, T., Pordea, A., Sardo, A., Wilson, YM, Knörr, L., Creus, M.,
Prost, J.-C., Schirmer, T. and Ward, TR (2011), OsO 4⋅ Streptavidin: A Tunable Hybrid
Catalyst for the Enantioselective cis-Dihydroxylation of Olefins. Angewandte Chemie ...
All 3 versions
[PDF] from uky.eduHD Chirra, T Sexton, D Biswal, LB Hersh… - Acta Biomaterialia, 2011 - Elsevier
The use of proteins for therapeutic applications requires the protein to maintain sufficient
activity for the period of in vivo treatment. Many proteins exhibit a short half-life in vivo and,
thus, require delivery systems for them to be applied as therapeutics. The relative ...
Related articles - All 3 versions
The use of proteins for therapeutic applications requires the protein to maintain sufficient
activity for the period of in vivo treatment. Many proteins exhibit a short half-life in vivo and,
thus, require delivery systems for them to be applied as therapeutics. The relative ...
Related articles - All 3 versions
M Lehnert, M Gorbahn, C Rosin, M Klein… - Langmuir, 2011 - ACS Publications
It is well-known that protein-modified implant surfaces such as TiO2 show a higher
bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major
role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant ...
Cited by 1 - Related articles - All 3 versions
It is well-known that protein-modified implant surfaces such as TiO2 show a higher
bioconductivity. Fibronectin is a glycoprotein from the extracellular matrix (ECM) with a major
role in cell adhesion. It can be applied on titanium oxide surfaces to accelerate implant ...
Cited by 1 - Related articles - All 3 versions
M Moreno-Guzmán, A González-Cortés… - Analytica chimica …, 2011 - Elsevier
Abstract A novel electrochemical immunosensor was developed for the determination of the
hormone prolactin. The design involved the use of screen-printed carbon electrodes and
streptavidin-functionalized magnetic particles. Biotynilated anti-prolactin antibodies were ...
Cited by 1 - Related articles - All 4 versions
Abstract A novel electrochemical immunosensor was developed for the determination of the
hormone prolactin. The design involved the use of screen-printed carbon electrodes and
streptavidin-functionalized magnetic particles. Biotynilated anti-prolactin antibodies were ...
Cited by 1 - Related articles - All 4 versions
S Inouye, JI Sato, S Sasaki… - Bioscience, biotechnology, and …, 2011 - J-STAGE
The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion
bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-
Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model ...
Related articles - All 5 versions
The fusion protein of streptavidin to aequorin (STA-AQ) was highly purified from inclusion
bodies in Escherichia coli cells and applied to a bioluminescent sandwich immunoassay. α-
Fetoprotein (AFP), which is a serological marker of liver cancer, was used as a model ...
Related articles - All 5 versions
M Lehnert, M Gorbahn, M Klein… - … Research Part A, 2011 - Wiley Online Library
Lehnert, M., Gorbahn, M., Klein, M., Al-Nawas, B., Köper, I., Knoll, W. and Veith, M.(2011),
Streptavidin-coated TiO2 surfaces are biologically inert: Protein adsorption and osteoblast
adhesion studies. Journal of Biomedical Materials Research Part A. doi: 10.1002/jbm. a. ...
Lehnert, M., Gorbahn, M., Klein, M., Al-Nawas, B., Köper, I., Knoll, W. and Veith, M.(2011),
Streptavidin-coated TiO2 surfaces are biologically inert: Protein adsorption and osteoblast
adhesion studies. Journal of Biomedical Materials Research Part A. doi: 10.1002/jbm. a. ...
J Strömqvist, L Nardo, O Broekmans, J Kohn… - The European Physical …, 2011 - Springer
Abstract The Biotin-Streptavidin complex is a widely studied system in biology and
biophysics, because of its extremely strong non-covalent binding affinity. The latter is often
exploited to link molecules to substrates or to one another. However, the details of the ...
Abstract The Biotin-Streptavidin complex is a widely studied system in biology and
biophysics, because of its extremely strong non-covalent binding affinity. The latter is often
exploited to link molecules to substrates or to one another. However, the details of the ...
T Matsumoto, S Sawamoto, T Sakamoto… - Journal of …, 2011 - Elsevier
Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and
streptavidin modification of proteins or small molecules is widely used for biotechnology tool.
Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We ...
Cited by 2 - Related articles - All 4 versions
Streptavidin is tetrameric protein which has tight and specific biotin binding affinity, and
streptavidin modification of proteins or small molecules is widely used for biotechnology tool.
Here, we demonstrate site-specific streptavidin-protein conjugation using enzymes. We ...
Cited by 2 - Related articles - All 4 versions
D Horak, A Spanova, J Tvrdikova… - European Polymer Journal, 2011 - Elsevier
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl
methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4
nanoparticles coated with oleic acid, produced monodisperse magnetic poly (2- ...
All 2 versions
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl
methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4
nanoparticles coated with oleic acid, produced monodisperse magnetic poly (2- ...
All 2 versions
X Jiang, K Chen… - Biosensors and Bioelectronics, 2011 - Elsevier
A novel electrochemical biosensor was developed for detecting short DNA oligonucleotide
of Bacillus thuringiensis (Bt) transgenic sequence based on Ag nanoparticle aggregates. To
fabricate this DNA biosensor, the thiol-modified capture DNA (cDNA) was first anchored ...
All 2 versions
A novel electrochemical biosensor was developed for detecting short DNA oligonucleotide
of Bacillus thuringiensis (Bt) transgenic sequence based on Ag nanoparticle aggregates. To
fabricate this DNA biosensor, the thiol-modified capture DNA (cDNA) was first anchored ...
All 2 versions
D Gizachew… - Chemical Biology & Drug Design, 2011 - Wiley Online Library
Protein–protein interactions control signaling, specific adhesion, and many other biological
functions. The three-dimensional structures of the interfaces and bound ligand can be
approached with transferred nuclear Overhauser effect spectroscopy NMR, which can be ...
Related articles - All 3 versions
Protein–protein interactions control signaling, specific adhesion, and many other biological
functions. The three-dimensional structures of the interfaces and bound ligand can be
approached with transferred nuclear Overhauser effect spectroscopy NMR, which can be ...
Related articles - All 3 versions
[HTML] from nih.govCE Chivers, AL Koner, ED Lowe… - Biochemical Journal, 2011 - ncbi.nlm.nih.gov
Abstract The interaction between SA (streptavidin) and biotin is one of the strongest non-
covalent interactions in Nature. SA is a widely used tool and a paradigm for protein–ligand
interactions. We previously developed a SA mutant, termed Tr (traptavidin), possessing a ...
Cited by 2 - Related articles - All 15 versions
Abstract The interaction between SA (streptavidin) and biotin is one of the strongest non-
covalent interactions in Nature. SA is a widely used tool and a paradigm for protein–ligand
interactions. We previously developed a SA mutant, termed Tr (traptavidin), possessing a ...
Cited by 2 - Related articles - All 15 versions
L Mao, R Yuan, Y Chai, Y Zhuo… - Analyst, 2011 - xlink.rsc.org
Abstract The potential controlling silver catalysis for Ru (bpy) 3 2+
electrochemiluminescence (ECL) signal at a special potential− 0.4 [similar] 1.25 V was
newly developed as the new ECL signal amplification strategy for ultrasensitive protein ...
Related articles - All 4 versions
Abstract The potential controlling silver catalysis for Ru (bpy) 3 2+
electrochemiluminescence (ECL) signal at a special potential− 0.4 [similar] 1.25 V was
newly developed as the new ECL signal amplification strategy for ultrasensitive protein ...
Related articles - All 4 versions
R Bruck, E Melnik, P Muellner, R Hainberger… - Biosensors and …, 2011 - Elsevier
We report the development of a Mach-Zehnder interferometer biosensor based on a high
index contrast polymer material system and the demonstration of label-free online
measurement of biotin–streptavidin binding on the sensor surface. The surface of the ...
Cited by 1 - Related articles - All 5 versions
We report the development of a Mach-Zehnder interferometer biosensor based on a high
index contrast polymer material system and the demonstration of label-free online
measurement of biotin–streptavidin binding on the sensor surface. The surface of the ...
Cited by 1 - Related articles - All 5 versions
M Kubota, K Yoshimoto, X Yuan… - Polymer journal, 2011 - nature.com
In the development of high-performance materials for biosensing and bioseparation, an effective
blocking treatment is important to decrease nonspecific adsorption onto the surfaces of
protein-immobilized substrates and to increase the dispersion stability of substrate ...
Cited by 1 - Related articles - All 4 versions
In the development of high-performance materials for biosensing and bioseparation, an effective
blocking treatment is important to decrease nonspecific adsorption onto the surfaces of
protein-immobilized substrates and to increase the dispersion stability of substrate ...
Cited by 1 - Related articles - All 4 versions
DM Czajkowsky, L Li, J Sun, J Hu… - ACS nano, 2011 - ACS Publications
Characterization of non-covalent interactions between nanometer-sized structures, such as
proteins, and solid surfaces is a subject of intense interest of late owing to the rapid
development of numerous solid materials for medical and technological applications. Yet ...
Characterization of non-covalent interactions between nanometer-sized structures, such as
proteins, and solid surfaces is a subject of intense interest of late owing to the rapid
development of numerous solid materials for medical and technological applications. Yet ...
J Fahrer, J Funk, M Lillich… - Naunyn-Schmiedeberg's archives of …, 2011 - Springer
Abstract The C2 toxin produced by Clostridium botulinum is a binary AB-type exotoxin
composed of the enzyme subunit C2I and the binding/translocation moiety C2II. After
proteolytic activation, C2IIa mediates the subsequent internalization of C2I into the cytosol ...
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Abstract The C2 toxin produced by Clostridium botulinum is a binary AB-type exotoxin
composed of the enzyme subunit C2I and the binding/translocation moiety C2II. After
proteolytic activation, C2IIa mediates the subsequent internalization of C2I into the cytosol ...
Cited by 2 - Related articles - All 4 versions
[PDF] from tkk.fiAP Eskelinen, A Kuzyk, TK Kaltiaisenaho… - Small, 2011 - Wiley Online Library
Eskelinen, A.-P., Kuzyk, A., Kaltiaisenaho, TK, Timmermans, MY, Nasibulin, AG, Kauppinen,
EI and Törmä, P., Assembly of Single-Walled Carbon Nanotubes on DNA-Origami
Templates through Streptavidin–Biotin Interaction. Small, n/a. doi: 10.1002/smll. 201001750
Cited by 5 - Related articles - All 3 versions
Eskelinen, A.-P., Kuzyk, A., Kaltiaisenaho, TK, Timmermans, MY, Nasibulin, AG, Kauppinen,
EI and Törmä, P., Assembly of Single-Walled Carbon Nanotubes on DNA-Origami
Templates through Streptavidin–Biotin Interaction. Small, n/a. doi: 10.1002/smll. 201001750
Cited by 5 - Related articles - All 3 versions
Y Xu, L Pharand, Q Wen, F Gonzaga, Y Li… - Colloid & Polymer …, 2011 - Springer
Abstract Compared are two approaches for the biotinylation of poly (N-isopropylacrylamide-
co-vinylacetic acid) microgels, 300-nm diameter, water swollen particles with a corona of
carboxyl groups. The biotinylated microgels are a platform for bioactive water-based ink. ...
Related articles - All 3 versions
Abstract Compared are two approaches for the biotinylation of poly (N-isopropylacrylamide-
co-vinylacetic acid) microgels, 300-nm diameter, water swollen particles with a corona of
carboxyl groups. The biotinylated microgels are a platform for bioactive water-based ink. ...
Related articles - All 3 versions
MP Raphael, CA Rappole, LK Kurihara… - Journal of …, 2011 - Springer
Abstract The pH-dependent binding affinity of either avidin or streptavidin for iminobiotin has
been utilized in studies ranging from affinity binding chromatography to dynamic force
spectroscopy. Regardless of which protein is used, the logarithmic dependence of the ...
Related articles - All 4 versions
Abstract The pH-dependent binding affinity of either avidin or streptavidin for iminobiotin has
been utilized in studies ranging from affinity binding chromatography to dynamic force
spectroscopy. Regardless of which protein is used, the logarithmic dependence of the ...
Related articles - All 4 versions
KH Lim, H Huang, A Pralle… - Biochemistry, 2011 - ACS Publications
Although streptavidin s high affinity for biotin has made it a widely used and studied
binding protein and labeling tool, its tetrameric structure may interfere with some assays. A
streptavidin mutant with a simpler quaternary structure would demonstrate a molecular ...
Cited by 1 - Related articles - All 2 versions
Although streptavidin s high affinity for biotin has made it a widely used and studied
binding protein and labeling tool, its tetrameric structure may interfere with some assays. A
streptavidin mutant with a simpler quaternary structure would demonstrate a molecular ...
Cited by 1 - Related articles - All 2 versions
A Vesel, K Elersic… - Vacuum, 2011 - Elsevier
Abstract Immobilization of the protein streptavidin to the surface of polymethyl methacrylate
(PMMA) polymer was studied by X-ray photoelectron spectroscopy (XPS) and atomic force
microscopy (AFM). Different protocols were used to attach streptavidin to the PMMA ...
Abstract Immobilization of the protein streptavidin to the surface of polymethyl methacrylate
(PMMA) polymer was studied by X-ray photoelectron spectroscopy (XPS) and atomic force
microscopy (AFM). Different protocols were used to attach streptavidin to the PMMA ...
BC Galarreta, PR Norton… - Langmuir, 2011 - ACS Publications
A two-dimensional array of gold nanotriangles inscribed onto glass coverslips were
optimized for the surface-enhanced Raman detection of streptavidin/biotin monolayer
assemblies. The nanostructures were fabricated by electron beam lithography, and its ...
Cited by 1 - Related articles - All 4 versions
A two-dimensional array of gold nanotriangles inscribed onto glass coverslips were
optimized for the surface-enhanced Raman detection of streptavidin/biotin monolayer
assemblies. The nanostructures were fabricated by electron beam lithography, and its ...
Cited by 1 - Related articles - All 4 versions
K Effenberger‐Neidnicht, S Breyer, K Mahal… - …, 2011 - Wiley Online Library
Effenberger-Neidnicht, K., Breyer, S., Mahal, K., Diestel, R., Sasse, F. and Schobert,
R.(2011), Cellular Localisation of Antitumoral 6-Alkyl Thymoquinones Revealed by an
Alkyne–Azide Click Reaction and the Streptavidin–Biotin System. ChemBioChem, 12: ...
Cited by 1 - Related articles - All 4 versions
Effenberger-Neidnicht, K., Breyer, S., Mahal, K., Diestel, R., Sasse, F. and Schobert,
R.(2011), Cellular Localisation of Antitumoral 6-Alkyl Thymoquinones Revealed by an
Alkyne–Azide Click Reaction and the Streptavidin–Biotin System. ChemBioChem, 12: ...
Cited by 1 - Related articles - All 4 versions
[HTML] from scielo.brH Wang, C Zhao… - Brazilian Journal of Microbiology, 2011 - SciELO Brasil
ABSTRACT Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical
step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific
identification target to develop a novel hybridization signal amplification method (HSAM) ...
Cached
ABSTRACT Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical
step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific
identification target to develop a novel hybridization signal amplification method (HSAM) ...
Cached
YJ Weng, FJ Jing, JY Chen… - Applied Surface Science, 2011 - Elsevier
Abstract Construction of heparinylated multilayer films on Ti-O via streptavidin/biotin
interaction was conducted in the present study. An organic layer of 3-
aminopropylphosphonic acid (APP) was first introduced on Ti–O by self-assembling, and ...
Abstract Construction of heparinylated multilayer films on Ti-O via streptavidin/biotin
interaction was conducted in the present study. An organic layer of 3-
aminopropylphosphonic acid (APP) was first introduced on Ti–O by self-assembling, and ...
[CITATION] How the biotin-streptavidin interaction was made even stronger: investigation via crystallography and a chimeric tetramer
Related articles
K Borchers, V Schönhaar, T Hirth… - Journal of Dispersion …, 2011 - Taylor & Francis
Aqueous inks containing the protein streptavidin (SAv) or nanoparticles carrying surface-
attached SAv were formulated for piezoelectric inkjet printing, using glycerol and 2-propanol
to adjust their dynamic viscosity and their surface tension by variation of concentration and ...
Aqueous inks containing the protein streptavidin (SAv) or nanoparticles carrying surface-
attached SAv were formulated for piezoelectric inkjet printing, using glycerol and 2-propanol
to adjust their dynamic viscosity and their surface tension by variation of concentration and ...
L Du, S Cheng… - Luminescence, 2011 - Wiley Online Library
A rapid and sensitive time-resolved fluoroimmunoassay (TR–FIA) based on the biotin–
streptavidin amplification system was developed for the determination of diethylstilbestrol
(DES). Europium-labelled streptavidin derivatives combined with europium and anhydride ...
Related articles - All 2 versions
A rapid and sensitive time-resolved fluoroimmunoassay (TR–FIA) based on the biotin–
streptavidin amplification system was developed for the determination of diethylstilbestrol
(DES). Europium-labelled streptavidin derivatives combined with europium and anhydride ...
Related articles - All 2 versions
LIU Jian, LIU Chun-xiu, LUO Jin-ping… - 哈尔滨工业大学 …, 2011 - 万方数据资源系统
LIU Ru-ping, TIAN Qing, CAI Xin-xia (State Key Lab of Transducer Technology, Institute of
Electronics, Chinese Academy of Sciences, Beijing 100080, China; Graduate University of
Chinese Academy of Sciences, Beijing 100080, China)
Cited by 1 - Related articles
LIU Ru-ping, TIAN Qing, CAI Xin-xia (State Key Lab of Transducer Technology, Institute of
Electronics, Chinese Academy of Sciences, Beijing 100080, China; Graduate University of
Chinese Academy of Sciences, Beijing 100080, China)
Cited by 1 - Related articles
T Rossel - 2011 - edoc.unibas.ch
Abstract Enzymes have been the subject of numerous studies and have been extensively
used to catalyse difficult chemical reactions. By extension, artificial metalloenzymes that
bridge the gap between organometallic catalysis and enzymatic catalysis are attracting ...
Cached
Abstract Enzymes have been the subject of numerous studies and have been extensively
used to catalyse difficult chemical reactions. By extension, artificial metalloenzymes that
bridge the gap between organometallic catalysis and enzymatic catalysis are attracting ...
Cached
K Hong Lim, I Hwang… - Biotechnology Progress - Wiley Online Library
Yeast surface display allows heterologously expressed proteins to be targeted to the exterior
of the cell wall and thus has a potential as a biotechnology platform. In this study, we report
the successful display of functional streptavidin on the yeast surface. Streptavidin binds ...
Related articles
Yeast surface display allows heterologously expressed proteins to be targeted to the exterior
of the cell wall and thus has a potential as a biotechnology platform. In this study, we report
the successful display of functional streptavidin on the yeast surface. Streptavidin binds ...
Related articles
[CITATION] Isotypes of 78 kDa streptavidin biotin-binding proteins (SBP) during initial germination of dark-grown pea (Pisum sativum L.) seedlings
Related articles - All 3 versions
刘剑， 刘春秀， 罗金平， 刘儒平， 田青… - Journal of Harbin Institute …, 2011 - cnki.com.cn
[摘要]: It is extremely important for bacteria detection in many fields, such as medical
diagnosis and food safety. In this paper, streptavidin functionalized quantum dots (SA-QDs),
as a nano-fluorescent probe, were used to attach with Escherichia coli (E. coli) for the ...
Cached
[摘要]: It is extremely important for bacteria detection in many fields, such as medical
diagnosis and food safety. In this paper, streptavidin functionalized quantum dots (SA-QDs),
as a nano-fluorescent probe, were used to attach with Escherichia coli (E. coli) for the ...
Cached
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