L Wang, Y Zhang, X Gao, Z Duan… - Journal of agricultural …, 2010 - ACS Publications
A sensitive biotin− streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA)
method was developed for the determination of chloramphenicol residues in milk. The
biotin− streptavidin system was applied to enhance the sensitivity. After optimization, the ...
Cited by 19 - Related articles - All 4 versions
A sensitive biotin− streptavidin amplified enzyme-linked immunosorbent assay (BA-ELISA)
method was developed for the determination of chloramphenicol residues in milk. The
biotin− streptavidin system was applied to enhance the sensitivity. After optimization, the ...
Cited by 19 - Related articles - All 4 versions
[HTML] from mcponline.orgHYH Kim, KA Tallman, DC Liebler… - Molecular & Cellular …, 2009 - ASBMB
Abstract HNE (4-hydroxynonenal), a byproduct of lipid peroxidation, reacts with nucleophilic
centers on proteins. A terminal alkynyl analog of HNE (alkynyl HNE, aHNE) serves as a
surrogate for HNE itself, both compounds reacting with protein amine and thiol functional ...
Cited by 11 - Related articles - All 8 versions
Abstract HNE (4-hydroxynonenal), a byproduct of lipid peroxidation, reacts with nucleophilic
centers on proteins. A terminal alkynyl analog of HNE (alkynyl HNE, aHNE) serves as a
surrogate for HNE itself, both compounds reacting with protein amine and thiol functional ...
Cited by 11 - Related articles - All 8 versions
[PDF] from hokudai.ac.jpR Kawamura, A Kakugo, Y Osada… - Nanotechnology, 2010 - iopscience.iop.org
Recently, a method was established for the formation of microtubule (MT) assemblies by an
active self-organization (AcSO) process, in which MTs were crosslinked during sliding
motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate ( ...
Cited by 11 - Related articles - All 11 versions
Recently, a method was established for the formation of microtubule (MT) assemblies by an
active self-organization (AcSO) process, in which MTs were crosslinked during sliding
motion on a kinesin-coated surface, and this was coupled with adenosine triphosphate ( ...
Cited by 11 - Related articles - All 11 versions
[HTML] from nih.govCE Chivers, E Crozat, C Chu, VT Moy… - Nature methods, 2010 - nature.com
Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur,
limiting its use in imaging, DNA amplification and nanotechnology. We identified a mutant
streptavidin, traptavidin, with more than tenfold slower biotin dissociation, increased ...
Cited by 8 - Related articles - All 9 versions
Streptavidin binds biotin conjugates with exceptional stability but dissociation does occur,
limiting its use in imaging, DNA amplification and nanotechnology. We identified a mutant
streptavidin, traptavidin, with more than tenfold slower biotin dissociation, increased ...
Cited by 8 - Related articles - All 9 versions
[HTML] from nih.govHA Ebhardt… - RNA, 2009 - rnajournal.cshlp.org
Abstract Here we present a simple and inexpensive gel-shift assay for the detection and
quantification of small RNAs. The assay is at least 5–10 times more sensitive than a
conventional Northern, and is highly scalable. Total RNA is first size purified to enrich the ...
Cited by 4 - Related articles - All 11 versions
Abstract Here we present a simple and inexpensive gel-shift assay for the detection and
quantification of small RNAs. The assay is at least 5–10 times more sensitive than a
conventional Northern, and is highly scalable. Total RNA is first size purified to enrich the ...
Cited by 4 - Related articles - All 11 versions
[CITATION] The C2-streptavidin delivery system promotes the uptake of biotinylated molecules in macrophages and T-leukemia cells
Cited by 4 - Related articles - All 3 versions
[PDF] from abbs.infoC Wang, G Yang, Z Luo… - Acta biochimica et …, 2009 - abbs.oxfordjournals.org
Abstract In this study, we developed a systematic evolution of ligands by exponential
enrichment (SELEX) method using a combination of magnetic beads immobilization and
flow cytometric measurement. As an example, the selection of streptavidin-specific ...
Cited by 3 - Related articles - All 8 versions
Abstract In this study, we developed a systematic evolution of ligands by exponential
enrichment (SELEX) method using a combination of magnetic beads immobilization and
flow cytometric measurement. As an example, the selection of streptavidin-specific ...
Cited by 3 - Related articles - All 8 versions
[HTML] from nih.govL Baugh, I Le Trong, DS Cerutti, S Gülich… - Biochemistry, 2010 - ACS Publications
We have identified a distal point mutation in streptavidin that causes a 1000-fold reduction in
biotin binding affinity without disrupting the equilibrium complex structure. The F130L
mutation creates a small cavity occupied by a water molecule; however, all neighboring ...
Cited by 3 - Related articles - All 4 versions
We have identified a distal point mutation in streptavidin that causes a 1000-fold reduction in
biotin binding affinity without disrupting the equilibrium complex structure. The F130L
mutation creates a small cavity occupied by a water molecule; however, all neighboring ...
Cited by 3 - Related articles - All 4 versions
T Tanaka, S Masunari, J Ishii, K Wakamura… - Journal of …, 2010 - Elsevier
Here we expand the yeast cell surface display system to display non-natural, functional
molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli
(BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through ...
Cited by 2 - Related articles - All 3 versions
Here we expand the yeast cell surface display system to display non-natural, functional
molecules. The short biotin acceptor peptide (BAP) sequence of biotin ligase from E. coli
(BirA) was genetically introduced to the N-terminus of the anchor protein, Flo428. Through ...
Cited by 2 - Related articles - All 3 versions
[HTML] from rsmjournals.comVHB Ho, KH Müller, NJ Darton… - Experimental …, 2009 - ebm.rsmjournals.com
Abstract A simple methodology for cell patterning has been developed that can potentially
be used to position different types of mammalian cells with high precision. In this method,
cell membrane proteins were first biotinylated and then bound to streptavidin ...
Cited by 3 - Related articles - All 7 versions
Abstract A simple methodology for cell patterning has been developed that can potentially
be used to position different types of mammalian cells with high precision. In this method,
cell membrane proteins were first biotinylated and then bound to streptavidin ...
Cited by 3 - Related articles - All 7 versions
JG Bruno - In Vitro Cellular & Developmental Biology-Animal, 2010 - Springer
Abstract Nucleic acid aptamers are regarded as rivals for antibodies and as such are being
investigated for their therapeutic potential. In the present work, it is shown that two different
high-affinity DNA aptamers developed previously by Ferreira et al. against MUC1 antigen ...
Cited by 2 - Related articles - All 5 versions
Abstract Nucleic acid aptamers are regarded as rivals for antibodies and as such are being
investigated for their therapeutic potential. In the present work, it is shown that two different
high-affinity DNA aptamers developed previously by Ferreira et al. against MUC1 antigen ...
Cited by 2 - Related articles - All 5 versions
T Kudo, M Ueda, H Konishi, H Kawashima… - Molecular Imaging and …, 2010 - Springer
Abstract Purpose We aimed to evaluate the feasibility of using streptavidin–biotin-based
pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor
(HIF)-1-active tumors. Procedures We used POS, a genetically engineered form of ...
Cited by 3 - Related articles - All 3 versions
Abstract Purpose We aimed to evaluate the feasibility of using streptavidin–biotin-based
pretargeting for positron emission tomography (PET) imaging of hypoxia-inducible factor
(HIF)-1-active tumors. Procedures We used POS, a genetically engineered form of ...
Cited by 3 - Related articles - All 3 versions
SP Rad'ko, SA Voronina, AV Gromov… - Bulletin of experimental …, 2009 - Springer
Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied
by the surface plasmon resonance method. Conjugation of the probe with streptavidin and
gold nanoparticles was shown to amplify the signal at similar concentrations of this probe ( ...
Cited by 1 - Related articles - All 5 versions
Hybridization of telomeric repeats with a complementary oligonucleotide probe was studied
by the surface plasmon resonance method. Conjugation of the probe with streptavidin and
gold nanoparticles was shown to amplify the signal at similar concentrations of this probe ( ...
Cited by 1 - Related articles - All 5 versions
K Hensley - Methods in molecular biology (Clifton, NJ), 2009 - Springer
Summary Oxidative posttranslational protein modifications occur as a normal process of cell
biology and to a greater extent during pathogenic conditions. The detection and quantitation
of protein oxidation has posed a continuing challenge to bioanalytical chemists because ...
Cited by 1 - Related articles - All 2 versions
Summary Oxidative posttranslational protein modifications occur as a normal process of cell
biology and to a greater extent during pathogenic conditions. The detection and quantitation
of protein oxidation has posed a continuing challenge to bioanalytical chemists because ...
Cited by 1 - Related articles - All 2 versions
MA Zhi-hong - Journal of Anhui Agricultural Sciences, 2009 - en.cnki.com.cn
Based on biotin-streptavidin enzyme-linked immunosorbent assay (ELISA), the detection
method on zearalenone ZEN-ELISA was established. The detection sensitivity of this
method was 0.8 ng/ml, and the variation coefficients of intra-assay and inter-assay were ...
Cited by 1 - Related articles - Cached
Based on biotin-streptavidin enzyme-linked immunosorbent assay (ELISA), the detection
method on zearalenone ZEN-ELISA was established. The detection sensitivity of this
method was 0.8 ng/ml, and the variation coefficients of intra-assay and inter-assay were ...
Cited by 1 - Related articles - Cached
CK Hsu… - Journal of Molecular Graphics and Modelling, 2010 - Elsevier
Wt streptavidin forms a domain swapped tetramer consisting of two native dimers. The role
of tetramerization has been studied previously and is known to contribute to biotin binding
by allowing the exchange of W120 between adjacent subunits. However, the role of dimer ...
Cited by 1 - Related articles - All 3 versions
Wt streptavidin forms a domain swapped tetramer consisting of two native dimers. The role
of tetramerization has been studied previously and is known to contribute to biotin binding
by allowing the exchange of W120 between adjacent subunits. However, the role of dimer ...
Cited by 1 - Related articles - All 3 versions
L No - img.creative-biomart.com
1. Howarth M., et al. (2006) Nat Methods. 3(4):267-73. 2. Holmberg A., et al (2005).
Electrophoresis. 26(3):501-10. ... Streptomyces avidinii, Recombinant(Streptavidin,
His-tagged) Expressed in E.coli Cat. No. Streptavidin-390S Lot. No. (See product label)
Related articles - View as HTML
1. Howarth M., et al. (2006) Nat Methods. 3(4):267-73. 2. Holmberg A., et al (2005).
Electrophoresis. 26(3):501-10. ... Streptomyces avidinii, Recombinant(Streptavidin,
His-tagged) Expressed in E.coli Cat. No. Streptavidin-390S Lot. No. (See product label)
Related articles - View as HTML
[PDF] B3 (Fab)-streptavidin Tetramer Has Higher Binding Avidity than B3 (scFv)-streptavidin Tetramer
Multivalent and multi-specific antibodies can provide valuable tools for bio-medical
research, diagnosis and therapy. In antigen-antibody interactions, the avidity of antibodies
depends on the affinity and the number of binding sites. 1 As artificial multivalent antibody ...
Related articles - View as HTML - All 5 versions
CX Xu, ZM Hu, JL Li… - Nan fang yi ke da xue xue bao= …, 2009 - ncbi.nlm.nih.gov
OBJECTIVE: To study the purification, refolding and bioactivity of streptavidin-tagged human
tumor necrosis factor-alpha (SA-TNF-alpha) bi-functional fusion protein. METHODS: SA-TNF-
alpha fusion protein was expressed in BL21 (DE3) host bacteria, purified using Ni-NTA ...
OBJECTIVE: To study the purification, refolding and bioactivity of streptavidin-tagged human
tumor necrosis factor-alpha (SA-TNF-alpha) bi-functional fusion protein. METHODS: SA-TNF-
alpha fusion protein was expressed in BL21 (DE3) host bacteria, purified using Ni-NTA ...
[HTML] from nih.govK Leung - 2009 - ncbi.nlm.nih.gov
Optical fluorescence imaging is increasingly being used to monitor biological functions of
specific targets in small animals (1-3). However, the intrinsic fluorescence of biomolecules
poses a problem when fluorophores that absorb visible light (350–700 nm) are used. Near ...
Related articles - All 3 versions
Optical fluorescence imaging is increasingly being used to monitor biological functions of
specific targets in small animals (1-3). However, the intrinsic fluorescence of biomolecules
poses a problem when fluorophores that absorb visible light (350–700 nm) are used. Near ...
Related articles - All 3 versions
S Yokota - Methods in molecular biology (Clifton, NJ), 2010 - Springer
Abstract Colloidal gold probes, including protein A-, IgG-F (ab') 2-, and streptavidin-labeled
gold particles, are useful tools for localization of antigens in cells and tissues by
immunoelectron microscopy (IEM). This chapter describes different methods for the ...
All 2 versions
Abstract Colloidal gold probes, including protein A-, IgG-F (ab') 2-, and streptavidin-labeled
gold particles, are useful tools for localization of antigens in cells and tissues by
immunoelectron microscopy (IEM). This chapter describes different methods for the ...
All 2 versions
H Su, YL Chen, SY Chen, B Wen… - Nan fang yi ke da xue …, 2009 - ncbi.nlm.nih.gov
OBJECTIVE: To obtain streptavidin-tagged human interleukin-15 (SA/hIL15) fusion protein
and evaluate its bioactivity. METHODS: pET24a-6His-SA-hIL-15 and pET32a-hIL-15-SA-
6His plasmids were constructed and expressed in BL 21 (DE3) host bacteria to generate ...
OBJECTIVE: To obtain streptavidin-tagged human interleukin-15 (SA/hIL15) fusion protein
and evaluate its bioactivity. METHODS: pET24a-6His-SA-hIL-15 and pET32a-hIL-15-SA-
6His plasmids were constructed and expressed in BL 21 (DE3) host bacteria to generate ...
C Oliver - Methods in molecular biology (Clifton, NJ), 2010 - ncbi.nlm.nih.gov
Biotin-avidin detection systems are widely used in both immunocytochemistry and molecular
biology. They take advantage of the high affinity of biotin, a low-molecular-weight vitamin, for
avidin, an egg-white protein. Because of the problem of nonspecific binding of avidin, ...
Biotin-avidin detection systems are widely used in both immunocytochemistry and molecular
biology. They take advantage of the high affinity of biotin, a low-molecular-weight vitamin, for
avidin, an egg-white protein. Because of the problem of nonspecific binding of avidin, ...
F PENG, X CHEN, F XU… - Food Science, 2009 - en.cnki.com.cn
PENG Fu-zhong1, CHEN Xue-lan1,*, XU Feng2, XIONG Yong-hua2 (1. College of Life
Science, Jiangxi Normal University, Nanchang 330022, China; 2. State Key Laboratory of
Food Science and Technology, Nanchang University, Nanchang 330047, China)
Cached
PENG Fu-zhong1, CHEN Xue-lan1,*, XU Feng2, XIONG Yong-hua2 (1. College of Life
Science, Jiangxi Normal University, Nanchang 330022, China; 2. State Key Laboratory of
Food Science and Technology, Nanchang University, Nanchang 330047, China)
Cached
L Wang… - Methods in enzymology, 2010 - Elsevier
Abstract In this chapter, we describe the preparation of cryo-EM specimens for random
spherically constrained (RSC) single-particle reconstruction of membrane proteins. The
specimen consists of liposomes into which the purified membrane protein is reconstituted ...
Related articles - All 5 versions
Abstract In this chapter, we describe the preparation of cryo-EM specimens for random
spherically constrained (RSC) single-particle reconstruction of membrane proteins. The
specimen consists of liposomes into which the purified membrane protein is reconstituted ...
Related articles - All 5 versions
[PDF] from biomedcentral.comJH Kim, T Chang, A Graham, KA Choo… - BMC …, 2010 - biomedcentral.com
Background Cell biologists face the need to rapidly analyse their proteins of interest in order
to gain insight into their function. Often protein purification, cellular localisation and Western
blot analyses can be multi-step processes, where protein is lost, activity is destroyed or ...
Related articles - All 6 versions
Background Cell biologists face the need to rapidly analyse their proteins of interest in order
to gain insight into their function. Often protein purification, cellular localisation and Western
blot analyses can be multi-step processes, where protein is lost, activity is destroyed or ...
Related articles - All 6 versions
PP Fa, Z Zhang, JL Li, ZM Hu… - Nan fang yi ke da xue xue …, 2010 - ncbi.nlm.nih.gov
OBJECTIVE: To obtain streptavidin-tagged human interleukin-21 (hIL21) fusion protein and
evaluate its bioactivities. METHODS: hIL21-SA-pET21 and pET24a-SA-hIL21 plasmids were
constructed and expressed in BL21 (DE3) host bacteria. The hIL21-SA and SA-hIL21 ...
OBJECTIVE: To obtain streptavidin-tagged human interleukin-21 (hIL21) fusion protein and
evaluate its bioactivities. METHODS: hIL21-SA-pET21 and pET24a-SA-hIL21 plasmids were
constructed and expressed in BL21 (DE3) host bacteria. The hIL21-SA and SA-hIL21 ...
AY Golovina, AA Bogdanov, OA Dontsova… - Biochimie, 2010 - Elsevier
Preparation of pure ribosomal subunits carrying lethal mutations is necessary for studying
every essential functional region of ribosomal RNA. Affinity purification via a tag, inserted
into rRNA proved to be procedure of choice for purification of such ribosomal subunits. ...
Related articles - All 5 versions
Preparation of pure ribosomal subunits carrying lethal mutations is necessary for studying
every essential functional region of ribosomal RNA. Affinity purification via a tag, inserted
into rRNA proved to be procedure of choice for purification of such ribosomal subunits. ...
Related articles - All 5 versions
MJ Navakouski, II Vashkevich… - Applied Biochemistry and …, 2009 - Springer
Abstract The effect of precipitation of complexes of streptavidin with biotinylated proteins
under conditions of simple (according to Mancini) and double (according to Ouchterlony)
radial diffusion in agar gel was studied. The position and form of precipitation lines ...
Related articles - All 5 versions
Abstract The effect of precipitation of complexes of streptavidin with biotinylated proteins
under conditions of simple (according to Mancini) and double (according to Ouchterlony)
radial diffusion in agar gel was studied. The position and form of precipitation lines ...
Related articles - All 5 versions
M Park, J Jose, S Thommes, JI Kim… - Enzyme and Microbial …, 2010 - Elsevier
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion
protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse
adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed ...
Related articles - All 3 versions
Streptavidin was expressed on the outer membrane of E. coli as a recombinant fusion
protein with an autotransporter domain called AIDA-I (adhesin involved in diffuse
adherence) using autodisplay technology. The autodisplay of streptavidin was confirmed ...
Related articles - All 3 versions
[PDF] from roche.comC No - e-labdoc.roche.com
Streptavidin from E. coli K12 was coupled to alkaline phosphatase (AP) from calf intestine
via heterobifunctional linkers. This conjugate was purified using column chromatography.
The final preparation is stabilized in 30 mM triethanolamine buffer, pH 7.6, 0.5% bovine ...
Related articles - All 5 versions
Streptavidin from E. coli K12 was coupled to alkaline phosphatase (AP) from calf intestine
via heterobifunctional linkers. This conjugate was purified using column chromatography.
The final preparation is stabilized in 30 mM triethanolamine buffer, pH 7.6, 0.5% bovine ...
Related articles - All 5 versions
A Agarwal… - Biophysical Journal, 2009 - adsabs.harvard.edu
Title: Velocity-dependence of Cargo Loading onto Molecular Shuttles Demonstrates the
Glue-like Character of Biotin/Streptavidin. Authors: Agarwal, Ashutosh; Hess, Henry. Publication:
Biophysical Journal, vol. 96, issue 3, pp. 317a-317a. Publication Date: 02/2009. ...
All 2 versions
Title: Velocity-dependence of Cargo Loading onto Molecular Shuttles Demonstrates the
Glue-like Character of Biotin/Streptavidin. Authors: Agarwal, Ashutosh; Hess, Henry. Publication:
Biophysical Journal, vol. 96, issue 3, pp. 317a-317a. Publication Date: 02/2009. ...
All 2 versions
T Matsumoto, S Sawamoto, T Sakamoto… - Journal of bioscience …, 2009 - ci.nii.ac.jp
... 論文名, 著者名, 著者所属, 刊行物名, ISSN, 巻, 号, ページ, 出版者, 参考文献, 出版年, 年から 年まで.
すべて CiNiiに本文あり CiNiiに本文あり、または連携サービスへのリンクあり. EP-P27 Site-specific
streptavidin modification using sortase(Section VII Enzyme and Protein Engineering). ...
Cached - All 2 versions
... 論文名, 著者名, 著者所属, 刊行物名, ISSN, 巻, 号, ページ, 出版者, 参考文献, 出版年, 年から 年まで.
すべて CiNiiに本文あり CiNiiに本文あり、または連携サービスへのリンクあり. EP-P27 Site-specific
streptavidin modification using sortase(Section VII Enzyme and Protein Engineering). ...
Cached - All 2 versions
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